BIO-INSECTICIDES Approximately 300 million people worldwide are affected by malaria and between 1 and 1.5 million people die from it every year. Previously extremely widespread, the malaria is now mainly confined to Africa, Asia and Latin America. The problems of controlling malaria in these countries are aggravated by inadequate health structures and poor socioeconomic conditions. The situation has become even more complex over the last few years with the increase in resistance to the drugs normally used to combat the parasite that causes the disease.     GREENEX™ is produced from a strain of Bacillus thuringiensis subspecies israelensis (Bti), a naturally occurring bacterium that produces a crystalline protein toxin (crystal) and a spore. The larval activity of GREENEX™ formulations and all other Bti formulations is due to the presence of the protein toxin. The spore has no larvicidal activity GREENEX™ formulations are unique and specifically manufactured to enhance the toxin's larvicidal effects and to ensure the toxin's biological stability. The formulations of GREENEX™ presently available are: (1) GREENEX™ xt Biological Larvicide Aqueous Suspension and (2) GREENEX™ 200G Corn Granule (5/8 and 10/14 mesh available).     GREENEX™ formulations are produced in the United States to strict manufacturing specifications, ensuring that the products are of highest quality and without any harmful contaminants. Bti formulations are FDA approved.  The potency of GREENEX™ is controlled within specific limits using a bioassay procedure based on toxicity to larvae of Aedes aegypti. Quality control bioassays are conducted prior to the release of each lot of product. Other analyses are conducted on each lot to assure that there are no microbial contaminants present in the formulations. EFFECT ON NON-TARGET ORGANISMS Environmental fate: GREENEX™ is naturally occurring and safe to the environment. It shows no cross resistance to chemical larvicides and is especially well suited for use in areas where there is demonstrated resistance to organophosphates or pyrethroids. GREENEX™ does not persist in soil or water. The protein crystals separate from the aquatic environment and settle to the bottom. They are deactivated by becoming food for other microorganisms or being adsorbed to soil or other organic particles. Spores do not recycle or regenerate in the field.     No toxicity has been observed against crustacean including copepod species, Gambusia, oysters, shrimp, crabs, mollusks, flatworms and amphibian. In addition to safety to non-target vertebrates and invertebrates, Bti-based larvicides have been used in large scale mosquito and black fly control programs for 20 years with no adverse effects on humans. FACTORS AFFECTING PERFORMANCE Water conditions such as temperature, organic matter content, chlorine, some inorganic salts, and pH may affect performance of GREENEX™ formulations. Larval feeding rates, subsequent ingestion of GREENEX™ protein and larval mortality rates are positively correlated with water temperature. Organic matter and algae represent an alternative food source for mosquito larvae; the higher labelled rates are therefore, required to assure control. Water habitats containing high levels of mosquito larvae require a greater dose of GREENEX™ for effective larval control.     Our Mission Bio-Solutions Corp. develop products in the biotechnology sector for heath and nutrition of animals as well as microbial insecticides, bio-insecticides. The range of products offered by our company responds to the precise needs of clients who wish to obtain cost effective and environmentally safe solutions to the problems created by insects. Agriculture and Aquatics are two target areas of our company. The mission of Bio-Solutions Corp. in the bio-insecticides field, is to preserve our planet’s ecosystem through the use of safe yet highly effective biological agents against insect pests. As leader in the industry, Bio-Solutions Corp. offers high quality products, which are very compatible with integrated pest management (IPM) while protecting the biodiversity of species.      Bio-insecticides The need for new effective products In biological control, the production of new highly efficient formulations is now necessary. The improvement in biological insecticides, particularly Bacillus thuringiensis (Bt) – based products, must be based on: • an increase of the residual toxicity • a better protection of the active ingredients against environmental factors • a reduction in application dosages • the reduction of environmental impacts in insect pest control programs The development of new preparations containing specific virus to target organisms are also being examined. To preserve its lead in the development of new methods to combat insect infestation, Bio-Solutions Corp. is intensifying its research and development activities in order to meet the projected demand for environmentally sound products in the future mainly concentrated in the countries of Africa. All these reasons favor the utilization of the high efficiency line of products developed by Bio-Solutions Corp.